A novel real - time fluorescent rt - pcr was established to detect pnrsv this technique uses a taqman probe to monitor in real time amplification of target genes , a taqman probe labeled by a quenching and fluorecenting was added to the pcr reaction buffer 另外还设计了taqman荧光探针，确定了检测条件和参数，建立了实时荧光rtwcr方法，该方法是指在pcr反应体系中加入带有荧光基团互补探针，如果有pcr反应（扩增） ，荧光信号就较大。
Correct clones were selected and plasmid dna was isolated and digested with saci and puvii . a dna fragment of about 2 . 1kb was purified and labeled by dig - 11dutp as probe . at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe . among them one clone contains human serum album dna by sequence 以pcr扩增的人血清白蛋白( hsa )基因片段为探针，从人的基因组文库中杂交筛选的阳性克隆中，经测序分析，有一个克隆含有全长hsadna ，用从其它的阳性克隆中选取两种dna片段，即dna修复基因hfen1和一段非编码大片段cit987sk - 384d8 ，与人hsadna一起，进行显微共注射，成功制备了转多基因小鼠。
Honsen l119 series afff fire extinguishing agents are one of the ideal replacer of halon and other poisonous extinguishing agents . they are the only fire product which is honoured with green environment label by china and national fire production industry . and it makes green fire production come to be true 宏信l119水成膜泡沫灭火剂是中国和国际消防行业唯一获得绿色环境标志的消防产品，正是“哈龙”和其他有害灭火剂的理想替代产品，它真正实现了绿色消防。
In this study , in order to examine the dynamics of tip [ ca2 + ] i together with the dynamics of tip - localized f - actin in vivo , a kind of double labeled material was constructed . the ca2 + and actin microfilaments of arabidopsis pollen tubes were labeled by cameleon and gfp - mtalin respectively 本研究以拟南芥花粉管为材料，通过转基因技术，将分别标记ca ~ ( 2 + )和微丝的两种荧光蛋白cameleon与gfp - mtalin在花粉管中同时表达，实现活体花粉管中ca ~ ( 2 + )与微丝的同时标记。
One was cloning , sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ) , the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot 为了鉴别诊断tgev与prcv及对tgev进行流行病学调查，本研究采用原核表达系统( gst融合表达系统)表达tgev纤突蛋白( s蛋白)中含有b和c抗原位点的多肽，并且制备了非放射性地高辛标记的核酸探针，通过斑点杂交( dot - blot )检测tgev核酸rna 。
Was multiplied and the tk gene was cloned . the cloned tk gene was retrieved by proper restrictive hemodynamics . the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin . then , the specificity and sensitivity of tk gene probe were detected with dot blot hybridization . the sequence of tk gene of nm98a strain was analysed . the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99 . 7 % 本研究中首次对iltv - nm98a株的tk基因进行了克隆和序列分析，结果表明： iltv - nm98a株tk基因的核苷酸序列与已发表的iltvtk基因的核苷酸序列具有高度的同源性，两者之间仅相差4个核苷酸，同源性高达99 . 7 ，从而证实了iltvtk基因是高度保守的，为iltvtk基因核酸探针的制备提供了有力的依据。
If i want to elucidatied the complax problem , i had to analysis it from many aspacts . in the historical context in china then . the transmit had the historical invialibility . and the evolution acted as the historical invialibility by accident . our tranditional culture and the science practice in ancientry society offered the theory chance for the transmit . finally , the evolution labeled by science 在当时中国的历史情境下，这种传播是有其历史必然性的。而进化论就偶然地充当了这种历史必然性。而我国的传统文化和古代社会的科学实践则为这种传播提供了理论契机和物质基础。
Thin sections of host leaf cells infected by bbwv - 2 isolate b935 , which were gold - labeled by antibodies of bbwv - 2 coat protein ( cp ) and vp37 , respectively , were prepared to elucidate the locations of vp37 in cell and possible function of vp37 and cp in cell to cell movement . observation in electron microscope showed that virus particles were presented not only in cytoplasma but also in chloroplast , while vp37 was existed only in cytoplasma and associated with tubular structure through the cell wall 为研究vp37在寄主细胞中的作用机制及其在细胞中的分布，通过胶体金间接标记6his - vp37兔抗血清，同时还标记了病毒的外壳蛋白单克隆抗体，对bbwv - 2分离物b935感染的病叶超薄切片的电子显微镜观察发现：病毒粒子除了聚集在胞质中，还存在于寄主的叶绿体内； vp37蛋白能在细胞壁上形成管状结构，在胞质中亦有分布。
In march 2004 the company is registered after holding water , held water to develop what upper reaches of houseboat , sea sees boat project to prepare to construct namely place , this project was labelled by bureau of tourism of boat hill city on december 15 , 2005 key of boat hill city ( characteristic ) travel builds a project 在2004年3月公司注册成立后，即成立了开发游艇、海上游览船项目的筹建处， 2005年12月15日该项目被舟山市旅游局列为舟山市重点（特色）旅游建设项目。
The his - tagged peacl - gfp purified from the supernatants could polymerize into green fluorescent filamentous structures with diameter , length and shape being identical to that of muscle f - actins , which could be labeled by tritc - phalloidin ( a specific agent for staining actin microfilaments ) , and were identified as having a 9 nm diameter by negative staining , corresponding with that of the muscle f - actins ( 7 - 10 nm ) . under polymerization conditions , his - tagged peacl - gfp polymerized with kinetics similar to those of skeleton muscle actin , that is , an obvious lag nucleation period at the beginning of polymerization and an s - like typical polymerization curve could be obtained . the critical concentration is 0 . 75 umol / l near to that of chicken muscle actin ( 0 . 56 umol / l ) under the same condition 荧光标记结合荧光显微观察表明：从可溶性上清中纯化的his - taggedpeac1 - gfp聚合形成的微丝不仅可以直接在荧光显微镜下观察，也可被微丝的特异标记物鬼笔环肽所标记，而且其直径、长度以及形态上与已知的聚合肌动蛋白荧光丝一致；电镜负染的结果进一步证实其直径为9nm ，与传统微丝直径相当( 7 ? 10nm ) ；聚合曲线有明显的停滞期，为典型的s型聚合曲线，聚合临界浓度为0 . 75 mol l ，这一结果与已有报道相似。
